Ox-LDL促進CD4+T細胞Notch3、Notch4的表達及其意義

? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 楊敬寧* ? 陳俊
? ? ? ? ? ? ? 【摘要】目的 探討氧化低密度脂蛋白(ox-LDL)對人CD4+T細胞Notch3、Notch4表達的影響。方法： 采用密度梯度離心法分離健康人外周血單個核細胞，分離淋巴細胞，經流式細胞術分選CD4+T細胞，然后分為空白對照組、低、中、高劑量組，加入濃度分別為0 mg/L、50 mg/L、100 mg/L、200 mg/L的ox-LDL培養(yǎng)72 h，流式細胞術檢測CD4+T細胞Notch3、Notch4分子的表達變化。結果 流式細胞術檢測顯示，空白對照組、低、中、高劑量組的Notch3表達分別為（3.42±0.36%）、（16.98±1.05%）、（9.83±0.52%）、（15.65±0.96%）。與空白對照組比較，低、中、高劑量組Notch4表達率明顯升高，差異有統(tǒng)計學意義(P<0.01)。空白對照組，低、中、高劑量組的Notch4表達分別為（9.54±0.72%）、（29.33±2.29%）、（13.58±1.43%）、（36.62±3.27%）。與空白對照組比較，低、高劑量組Notch4表達率明顯增高，差異有統(tǒng)計學意義(P<0.01)。結論 ox-LDL能明顯促進人外周血CD4+T細胞Notch3、Notch4的表達，可能與T細胞分化的精細調節(jié)和炎癥反應的負調節(jié)有關，參與動脈粥樣硬化的發(fā)生、發(fā)展。

【關鍵詞】 氧化低密度脂蛋白；動脈粥樣硬化；Notch?；CD4+T細胞

Ox-LDL promotes the expression and significance of Notch3 and Notch4 in CD4+T cells.Yang Jingning,Chen Jun.Hubei Medical University, Shiyan 442000,Hubei,China.

【Abstract】Objective ?To observe the effect of oxidized low-density lipoprotein (ox-LDL) on the expression of Notch3 and Notch4 in human peripheral blood CD4+T cells. Methods?Density gradient centrifugation was used to separate the peripheral blood mononuclear cells from healthy people, and the CD4+?T cells were sorted by flow cytometry. T cells were divided into four groups: the blank control group, low, medium and high concentration group, and coculture with ox-LDL.The concentration of ox-LDL was 0 mg/L, 50 mg/L, 100 mg/L and 200 mg/L respectively. The expression of Notch3 and Notch4 in CD4+T cells was detected by flow cytometry in each group after 72 h. Results The results revealed that the expression of Notch3 in each group was 3.42±0.36%, 16.98±1.05%, 9.83±0.52% and 15.65±0.96% respectively. Compared with the blank control group, the expression of Notch 3 in other groups were significantly increased, the difference was statistically significant (P<0.01). The expression of Notch 4 in each group was 9.54±0.72%, 29.33±2.29%, 13.58±1.43% and 36.62±3.27% respectively. Compared with the blank control group, the expression of Notch 4 was significant increased，and the differences were statistically significant (P<0.01). Conclusions ?Ox-LDL can significantly promote the expression of Notch 3 and Notch 4 in human peripheral blood CD4+T cells, which may be related to the fine regulation of T cell differentiation and the negative regulation of inflammatory reaction, and is involved in the occurrence and development of atherosclerosis.

【Key words】Oxidized low density lipoprotein ; Atherosclerosis; Notch; CD4+T cell